Prohibitin antibody (70R-5543)

Rabbit polyclonal Prohibitin antibody raised against the C terminal of PHB

Synonyms Polyclonal Prohibitin antibody, Anti-Prohibitin antibody, PHB antibody
Specificity Prohibitin antibody was raised against the C terminal of PHB
Cross Reactivity Human,Mouse,Rat
Applications IHC, WB
Immunogen Prohibitin antibody was raised using the C terminal of PHB corresponding to a region with amino acids AEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLS
Assay Information Prohibitin Blocking Peptide, catalog no. 33R-1146, is also available for use as a blocking control in assays to test for specificity of this Prohibitin antibody

Images

Immunohistochemical staining using Prohibitin antibody (70R-5543)

Prohibitin antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Epithelial cells of fundic gland (arrows) in Human Stomach. Magnification is at 400X

Specifications

Host Rabbit
Method of Purification Affinity purified
Molecular Weight 30 kDa (MW of target protein)
Form & Buffer Lyophilized powder. Add 50ul distilled water for a 1mg/ml concentration of PHB antibody in PBS
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1-2 ug/ml; IHC: 4-8 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles.

General Information

Biological Significance Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

References
Add a Paper

Tanaka Y, Fujii W, Hori H, Kitagawa Y, Ozaki K.

Relationship between coumarin-induced hepatocellular toxicity and mitochondrial function in rats

Food Chem Toxicol

Volume: 90 Page: 1-9 DOI: 10.1016/j.fct.2016.01.007

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Images

  • Immunohistochemical staining using Prohibitin antibody (70R-5543) | Prohibitin antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Epithelial cells of fundic gland (arrows) in Human Stomach. Magnification is at 400X
  • Western Blot analysis using Prohibitin antibody (70R-5543) | Prohibitin antibody (70R-5543) used at 1-2 ug/ml to detect target protein.

Availability: In stock

Price: $375.00
Size: 50 ug
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