Protein A antibody (FITC) (60C-CF0601)

Goat polyclonal Protein A antibody (FITC) conjugated

Synonyms Polyclonal Protein A antibody, Anti-Protein A antibody
Specificity Staphylococcus aureus protein A
Cross Reactivity To be determined by end-user
Applications FC, IF
Immunogen Protein A antibody (FITC) was raised in goat using protein A from Staphylococcus aureus as the immunogen.

Specifications

Host Goat
Method of Purification Protein A antibody (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Tag/Conjugate FITC
Form & Buffer Liquid in 0.01M Sodium Phosphate, 0.14M NaCl, pH 7.4 with 10mg/ml BSA (IgG and Protease free) and 0.01% Thimerosol

Usage & Assay Information

Usage Recommendations FC: 1:2,000-1:10,000, IF: 1:500-1:2,500

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.

General Information

Biological Significance Protein A is a 40-60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It binds proteins from many of mammalian species, most notably IgGs. It binds with the Fc region of immunoglobulins through interaction with the heavy chain.

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Availability: In stock

Price: $245.00
Size: 100 ul
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