Protein A (FITC) (65C-CE0402)

Immunoglobulin-binding bacterial FITC conjugated Protein A solution

Species Bacterial
Protein Type Binding Protein
Applications ELISA, FC, IF

Specifications

Method of Purification Protein A (FITC) was purified by Protein A affinity chromatography.
Tag/Conjugate FITC
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Protein A is a 40-60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It binds proteins from many of mammalian species, most notably IgGs. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. The result of this type of interaction is that, in serum, the bacteria will bind IgG molecules in the wrong orientation (in relation to normal antibody function) on their surface which disrupts opsonization and phagocytosis.

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Availability: In stock

Price: $323.00
Size: 5 mg
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