Protocols - Experimental Procedure for Research Applications

Click to download our selection of general protocols below:

Immunofluorescence Protocol

Our Immunofluorescence protocol provides step by step instructions for performing IF. This protocol provides details for preparation of cultured cell lines, paraffin sections and frozen/cryostat sections and the procedure for immunostaining.

Immunohistochemistry Protocol

Our Immunohistochemistry protocol outlines the basic procedure for performing IHC in easy to follow steps. This protocol covers IHC frozen sections, paraffin sections and the procedure for immunoenzyme staining.

Antigen Retrieval Protocol

Our Antigen Retrieval Protocol describes both Enzymatic and Heat Mediated (Microwave) methods. Ste-by-step instructions detail the requirements and actions for Antigen Retrieval.

Flow Cytometry Protocol

Our Flow Cytometry protocol outlines instructions on how to fix, permeabilize, and incubate your cells with primary/secondary antibodies for obtaining optimal FC results.

Immunocytochemistry Protocol

Our Immunocytochemistry protocol outlines some basic principals for fixing and staining cells, in addition to providing some links to some of our specific products which may be useful to you.

Western Blot Protocol

We understand that western blotting can be challenging at times, and our guidelines aim to standardize blocking procedures and help you to customize your own blotting assays.

Immunoprecipitation Protocol

The IP protocol outlines a simple method outlining standard procedures involved in immunoprecipitation assays. Watch out for our more detailed protocol coming very soon!

ELISA Protocol

A successful ELISA assay is dependent on careful and patient optimization. We recommend that our protocol should be used as a guide for scientists in developing a standard format for ELISA, and parameters adjusted and optimized based on experience.

ELISA Optimization Protocol

This protocol outlines the procedure for performing Checkerboard Titration (CBT), a key feature in the optimization of ELISA. We have included information on both Direct and Indirect ELISA CBT, reproduced with thanks to

Elispot Protocol

The Enzyme-linked immunosorbent spot (ELISPOT) assay is a common method for monitoring immune responses in humans and animals. At appropriate conditions the ELISPOT assay allows visualization of the secretory product of individual activated or responding cells. Each spot that develops in the assay represents a single reactive cell. Thus, the ELISPOT assay provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

Dot Blot Protocol

This Dot Blot protocol has been used successfully by many researchers and is one of the most straight forward protocols we have found.

Use of Blocking Peptides as Controls

Fitzgerald Industries has a portfolio of over 10,000 antibody/blocking peptide pairs and this protocol is a fool-proof tried and tested method for any antibody specificity assay.

Colloidal Gold Assays

We specialize in colloidal gold and we have over 20 years experience in working with customers in finding the right colloidal gold products for their assays. This detailed handbook has been passed onto hundreds of customers who have used this as a reference point for producing very successful lateral flow products for the commercial market.

PolyHRP Assays

Did you know that PolyHRP assays are a highly sensitive enhanced detection method applicable to an array of applications. Another highly sucessful and specialized area of our business is our expertise in providing PolyHRP products.We have produced a very detailed handbook on the advantages, uses, and products we provide for PolyHRP assays.

If you have any protocols or usage recommendations on any of our products that you would like to share with other scientists, please feel free to submit them to us at [email protected], and we will be delighted to post them for you.