PSEM3 protein (His tag) (80R-2044)
Recombinant human PSEM3 protein (His tag)
|Synonyms||PA28G protein, PSEM 3 protein, Proteasome activator complex subunit 3 isoform 1 protein, PSEM-3 protein, PA28-gamma protein, PSEM-3, PSEM 3, PSEM3, REG-GAMMA protein, Ki protein|
SDS-PAGE analysis of PSEM3 protein (His tag) (80R-2044)
SDS-PAGE gel analysis of PSEM3 protein (His tag) where the figure is annotated with amount of protein run on the gel.
|Residues||1-254 amino acids: MGSSHHHHHH SSGLVPRGSH MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY|
|Purity||> 90% pure|
|Molecular Weight||31.7 kDa (274aa), confirmed by MALDI-TOF|
|Form & Buffer||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl|
Usage & Assay Information
|Usage Recommendations||PSEM3 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.|
Storage & Safety
|Storage||Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||PSME3 (Proteasome activator complex subunit 3) belongs to the PA28 family. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSEM3 facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. Recombinant human PSME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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