PSEM3 protein (His tag) (80R-2044)

Recombinant human PSEM3 protein (His tag)

Synonyms PA28G protein, PSEM 3 protein, Proteasome activator complex subunit 3 isoform 1 protein, PSEM-3 protein, PA28-gamma protein, PSEM-3, PSEM 3, PSEM3, REG-GAMMA protein, Ki protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of PSEM3 protein (His tag) (80R-2044)

SDS-PAGE gel analysis of PSEM3 protein (His tag) where the figure is annotated with amount of protein run on the gel.

Specifications

Residues 1-254 amino acids: MGSSHHHHHH SSGLVPRGSH MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY
Expression System E.coli
Purity > 90% pure
Molecular Weight 31.7 kDa (274aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations PSEM3 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance PSME3 (Proteasome activator complex subunit 3) belongs to the PA28 family. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSEM3 facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. Recombinant human PSME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • SDS-PAGE analysis of PSEM3 protein (His tag) (80R-2044) | SDS-PAGE gel analysis of PSEM3 protein (His tag) where the figure is annotated with amount of protein run on the gel.

Availability: In stock

Price: $270.00
Size: 50 ug
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