PSMA1 protein (His tag) (80R-1995)

Recombinant human PSMA1 protein (His tag)

Synonyms PSMA-1, Proteasome subunit alpha type-1 isoform 2 protein, PSMA-1 protein, PROS30 protein, PSMA 1 protein, PSMA 1, PSMA1, HC2 protein, NU protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PSMA1 protein (His tag) (80R-1995)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-263 amino acids: MGSSHHHHHH SSGLVPRGSH MGSMFRNQYD NDVTVWSPQG RIHQIEYAME AVKQGSATVG LKSKTHAVLV ALKRAQSELA AHQKKILHVD NHIGISIAGL TADARLLCNF MRQECLDSRF VFDRPLPVSR LVSLIGSKTQ IPTQRYGRRP YGVGLLIAGY DDMGPHIFQT CPSANYFDCR AMSIGARSQS ARTYLERHMS EFMECNLNEL VKHGLRALRE TLPAEQDLTT KNVSIGIVGK DLEFTIYDDD DVSPFLEGLE ERPQRKAQPA QPADEPAEKA DEPMEH
Expression System E.coli
Purity > 95% pure
Molecular Weight 32 kDa (286aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations PSMA1 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance PSMA1 is a prosomal protein involved in a nonlysosomal ATP/ubiquitin-dependent proteolytic pathway. The protein is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. This protein highly expressed in prostate epithelium. Recombinant human PSMA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PSMA1 protein (His tag) (80R-1995) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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