PSMA5 protein (His tag) (80R-2078)

Recombinant human PSMA5 protein (His tag)

Synonyms PSMA5, PSC5 protein, ZETA protein, PSMA 5 protein, Proteasome subunit alpha type-5 protein, PSMA-5 protein, PSMA 5, PSMA-5
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PSMA5 protein (His tag) (80R-2078)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-241 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMFLT RSEYDRGVNT FSPEGRLFQV EYAIEAIKLG STAIGIQTSE GVCLAVEKRI TSPLMEPSSI EKIVEIDAHI GCAMSGLIAD AKTLIDKARV ETQNHWFTYN ETMTVESVTQ AVSNLALQFG EEDADPGAMS RPFGVALLFG GVDEKGPQLF HMDPSGTFVQ CDARAIGSAS EGAQSSLQEV YHKSMTLKEA IKSSLIILKQ VMEEKLNATN IELATVQPGQ NFHMFTKEEL EEVIKDI
Expression System E.coli
Purity > 90% pure
Molecular Weight 30.5 kDa (277aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance PSMA5 (Proteasome subunit alpha type-5) belongs to the peptidase T1A family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMA5 has been shown to interact with PLK1. This peptidase has a very broad specificity for cleavage of peptide bonds. Recombinant human PSMA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PSMA5 protein (His tag) (80R-2078) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 10 ug
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