PSMB3 protein (His tag) (80R-2047)

Recombinant human PSMB3 protein (His tag)

Synonyms PSMB-3, PSMB3, PSMB 3 protein, Proteasome subunit beta type-3 protein, HC10-II protein, PSMB 3, PSMB-3 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of PSMB3 protein (His tag) (80R-2047)

SDS-PAGE gel analysis of PSMB3 protein (His tag) where the figure is annotated with amount of protein run on the gel.

Specifications

Residues 1-205 amino acids: MGSSHHHHHH SSGLVPRGSH MSIMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP DHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL KARMD
Expression System E.coli
Purity > 90% pure
Molecular Weight 25.1 kDa (225aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl
Concentration 0.25 mg/ml

Usage & Assay Information

Usage Recommendations PSMB3 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.. Recombinant human PSMB3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • SDS-PAGE analysis of PSMB3 protein (His tag) (80R-2047) | SDS-PAGE gel analysis of PSMB3 protein (His tag) where the figure is annotated with amount of protein run on the gel.

Availability: In stock

Price: $365.00
Size: 100 ug
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