PSMB5 protein (His tag) (80R-1609)
Purified recombinant Human PSMB5 protein
Overview
Overview
| Synonyms | PSMB 5 protein, PSMB-5 protein, Proteasome (prosome macropain) subunit beta type 5. protein, MGC104214 protein, PSMB-5, LMPX protein, MB1 protein, PSMB 5, X., PSMB5 |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of PSMB5 protein (His tag) (80R-1609)
Figure annotation denotes ug of protein loaded and % gel used.
PSMB5 protein (His tag)
Coomassie Blue stained SDS-PAGE of PSMB5 protein (His tag) (80R-1609)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 60-263 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P |
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| Expression System | E.coli |
| Purity | > 90% pure |
| Molecular Weight | 26.7 kDa (241aa), confirmed by MALDI-TOF |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 5mM DTT and 0.2M NaCl. |
| Concentration | 0.25 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | PSMB5 is a member of the proteasome B-type family that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). PSMB5 has an ATP dependent proteolytic activity and involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway. Recombinant human PSMB5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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