PSMB5 protein (His tag) (80R-1609)
Purified recombinant Human PSMB5 protein
|Synonyms||PSMB 5 protein, PSMB-5 protein, Proteasome (prosome macropain) subunit beta type 5. protein, MGC104214 protein, PSMB-5, LMPX protein, MB1 protein, PSMB 5, X., PSMB5|
Coomassie Blue stained SDS-PAGE of PSMB5 protein (His tag) (80R-1609)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||60-263 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P|
|Purity||> 90% pure|
|Molecular Weight||26.7 kDa (241aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 5mM DTT and 0.2M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PSMB5 is a member of the proteasome B-type family that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). PSMB5 has an ATP dependent proteolytic activity and involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway. Recombinant human PSMB5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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