PSMC5 antibody (10R-1411)

Mouse monoclonal PSMC5 antibody

Synonyms Monoclonal PSMC5 antibody, Anti-PSMC5 antibody, TBP10 antibody, PSMC-5 antibody, PSMC-5, p45 antibody, PSMC 5, S8 antibody, SUG1 antibody, p45/SUG antibody, PSMC 5 antibody, TRIP1 antibody, 26S protease regulatory subunit 8 antibody, PSMC5
Specificity Human PSMC5
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen PSMC5 antibody was raised in mouse using recombinant Human Proteasome (Prosome, Macropain) 26S Subunit, Atpase, 5 (Psmc5)

Images

Immunofluorescent staining using PSMC5 antibody (10R-1411)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-PSMC5 antibody.

Specifications

Host Mouse
Clone 2837C4a
Isotype IgG1
Method of Purification PSMC5 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer PSMC5 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a nonlysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity.

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Images

  • Immunofluorescent staining using PSMC5 antibody (10R-1411) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-PSMC5 antibody.
  • Western Blot analysis of immunized recombinant protein using PSMC5 antibody (10R-1411)

Availability: In stock

Price: $190.00
Size: 50 ug
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