pykF protein (His tag) (80R-2244)

Purified recombinant E.coli pykF Protein (His tag)

Synonyms JW1666 protein, Pyruvate kinase I protein, ECK1672 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of pykF protein (His tag) (80R-2244)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTD RVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL
Expression System E.coli
Purity > 95% pure
Molecular Weight 53.3 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer, pH 8.0 containing 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance pykF is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia.

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Images

  • Coomassie Blue stained SDS-PAGE of pykF protein (His tag) (80R-2244) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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