RAB13 protein (His tag) (80R-1883)
Purified recombinant RAB13 protein
|Synonyms||GIG4 protein, RAB-13, RAB-13 protein, RAB13, RAB 13, RAB 13 protein|
Coomassie Blue stained SDS-PAGE of RAB13 protein (His tag) (80R-1883)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-200 amino acids: MGSSHHHHHH SSGLVPRGSH MAKAYDHLFK LLLIGDSGVG KTCLIIRFAE DNFNNTYIST IGIDFKIRTV DIEGKKIKLQ VWDTAGQERF KTITTAYYRG AMGIILVYDI TDEKSFENIQ NWMKSIKENA SAGVERLLLG NKCDMEAKRK VQKEQADKLA REHGIRFFET SAKSSMNVDE AFSSLARDIL LKSGGRRSGN GNKPPSTDLK TCDKKNTNKC|
|Purity||> 85% pure|
|Molecular Weight||24.7kDa (220aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 20% glycerol, 200mM NaCl and 1mM EDTA.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||RAB13 (Ras-related protein Rab-13) belongs to the small GTPase superfamily. Members of the RAB protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. RAB proteins are also an integral part of endocytic pathways. The GTPase Rab13 regulates the assembly of functional epithelial tight junctions (TJs). It is expressed at all stages of preimplantation development. Recombinant human RAB13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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