RAB25 antibody (10R-2236)

Mouse monoclonal RAB25 antibody

Synonyms Monoclonal RAB25 antibody, Anti-RAB25 antibody, RAB-25 antibody, RAB 25 antibody, RAB25, RAB 25, RAB-25, RAB 25 antibody, RAB-25 antibody
Cross Reactivity Human, Mouse
Applications ELISA, IF, IHC, WB
Immunogen RAB25 antibody was raised in Mouse using a purified recombinant fragment of RAB25 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using RAB25 antibody (10R-2236) (RAB25 antibody)

FC analysis of NIH/3T3 cells using Rab25 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 3F12
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance RAB proteins, such as RAB25, are members of the RAS superfamily of small GTPases that are involved in membranetrafficking. Members of the RAB11 subfamily, including RAB25, control the return of internalized membrane-associatedmoieties to the cell surface.

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Images

  • Flow Cytometric assay using Western Blot analysis using RAB25 antibody (10R-2236) (RAB25 antibody) | FC analysis of NIH/3T3 cells using Rab25 antibody (green) and negative control (purple).
  • Western Blot analysis using RAB25 antibody (10R-2236) | Western Blot showing Rab25 antibody used against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
  • Immunohistochemical staining using RAB25 antibody (10R-2236) | Immunohistochemical analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) using Rab25 antibody with DAB staining.
  • Immunofluorescent staining using Western Blot analysis using RAB25 antibody (10R-2236) (RAB25 antibody) | Immunofluorescence analysis of A549 cells using RAB25 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
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