RAB32 protein (His tag) (80R-2000)

Recombinant human RAB32 protein (His tag)

Synonyms RAB32, RAB-32, Ras-related protein Rab-32 protein, RAB 32, RAB-32 protein, RAB 32 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of RAB32 protein (His tag) (80R-2000)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-225 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMAGGGA GDPGLGAAAA PAPETREHLF KVLVIGELGV GKTSIIKRYV HQLFSQHYRA TIGVDFALKV LNWDSRTLVR LQLWDIAGQE RFGNMTRVYY KEAVGAFVVF DISRSSTFEA VLKWKSDLDS KVHLPNGSPI PAVLLANKCD QNKDSSQSPS QVDQFCKEHG FAGWFETSAK DNINIEEAAR FLVEKILVNH QSFPNEENDV DKIKLDQETL RAENKSQCC
Expression System E.coli
Purity > 90% pure
Molecular Weight 27.6 kDa (249aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 200mM NaCl, 2mM EDTA
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations RAB32 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance RAB32 belongs to the small GTPase superfamily. RAB32 modulates ER calcium handling and disrupts the specific enrichment of calnexin on the MAM (mitochondria-associated membrane), while not affecting the ER distribution of protein-disulfide isomerase and mitofusin-2. Also, RAB32 determines the targeting of PKA (cAMP-dependent protein kinase) to mitochondrial and ER membranes and through its overexpression or inactivation increases the phosphorylation of Bad and of Drp1. Through a combination of its functions as a PKA-anchoring protein and a regulator of MAM properties, the activity and expression level of RAB32 determine the speed of apoptosis onset. Recombinant human RAB32 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of RAB32 protein (His tag) (80R-2000) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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