RAB3A protein (His tag) (80R-1864)
Purified recombinant RAB3A protein
|Synonyms||RABA 3 protein, member RAS oncogene family protein, RABA 3, RAB3A protein, RAB3A, RABA-3, RABA-3 protein, Ras-related protein Rab-3A protein|
Coomassie Blue stained SDS-PAGE of RAB3A protein (His tag) (80R-1864)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-220 amino acids: MGSSHHHHHH SSGLVPRGSH MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NAVQDWSTQI KTYSWDNAQV LLVGNKCDME DERVVSSERG RQLADHLGFE FFEASAKDNI NVKQTFERLV DVICEKMSES LDTADPAVTG AKQGPQLSDQ QVPPHQDCAC|
|Purity||> 90% pure|
|Molecular Weight||27.1 kDa (240aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Rab3A belongs to the small GTPase superfamily, Rab family. It is a small, Ras-like GTPase expressed in neuroendocrine cells, in which it is associated with secretory vesicle membranes and regulates exocytosis. This protein could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. Recombinant human RAB3A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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