Rabbit anti Human IgG (40C-CB0950)

Rabbit anti Human IgG secondary antibody

Synonyms Rabbit anti Human Immunoglobulin G secondary antibody
Specificity Human IgG
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Rabbit anti-human IgG was raised in rabbit using human IgG F(c) fragment as the immunogen.

Images

Western blot analysis using Rabbit anti Human IgG (40C-CB0950)

Western Blot of Human Fc antibody. Lane 1: Human Fc. Lane 2: none. Load: 100 ng per lane. Human Fc antibody at 1:1,000 for overnight at 4 deg C. DyLight649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Human Fc. Other band(s): Human Fc splice variants and isoforms.

Specifications

Host Rabbit
Method of Purification Rabbit anti Human IgG was purified by affinity chromatography.
Form & Buffer 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
Concentration Batch dependent - please inquire should you have specific requirements

Usage & Assay Information

Usage Recommendations ELISA: 1:375,000, IHC: 1:1,000-1:6,000, WB: 1:3,000-1:30,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.

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Images

  • Western blot analysis using Rabbit anti Human IgG (40C-CB0950) | Western Blot of Human Fc antibody. Lane 1: Human Fc. Lane 2: none. Load: 100 ng per lane. Human Fc antibody at 1:1,000 for overnight at 4 deg C. DyLight649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Human Fc. Other band(s): Human Fc splice variants and isoforms.

Availability: In stock

Price: $275.00
Size: 5 mg
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