Rabbit anti Mouse IgG1 (Alk Phos) (43C-CB5126)

Rabbit anti Mouse IgG1 secondary antibody (Alk Phos)

Synonyms Rabbit anti Mouse Immunoglobulin G1 secondary antibody (Alk Phos)
Specificity Murine IgG1
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Rabbit anti-mouse IgG1 (Alk Phos) was raised in rabbit using murine IgG1 heavy chain as the immunogen.

Images

Western blot analysis using Rabbit anti Mouse IgG1 (Alk Phos) (43C-CB5126)

Western Blot of Mouse IgG1 Secondary antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. none. Mouse IgG1 Secondary antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4 deg C. Block: Blocking buffer for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Size: 55 kDa for Mouse IgG1.

Specifications

Host Rabbit
Method of Purification Rabbit anti Mouse IgG1 (Alk Phos) was purified by affinity chromatography.
Tag/Conjugate Alkaline Phosphatase
Form & Buffer 0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:5,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500

Storage & Safety

Storage Store at 4 deg C. DO NOT FREEZE.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).

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Images

  • Western blot analysis using Rabbit anti Mouse IgG1 (Alk Phos) (43C-CB5126) | Western Blot of Mouse IgG1 Secondary antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. none. Mouse IgG1 Secondary antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4 deg C. Block: Blocking buffer for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Size: 55 kDa for Mouse IgG1.
  • Dot Blot analysis using Rabbit anti Mouse IgG1 (Alk Phos) (43C-CB5126) | Dot Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 for one hour at RT. Block: MB-070 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.

Availability: In stock

Price: $365.00
Size: 1 mg
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