Rabbit anti Mouse IgG1 (rhodamine) (43C-CB5122)

Rabbit anti Mouse IgG1 secondary antibody (Rhodamine)

Synonyms Rabbit anti Mouse Immunoglobulin G1 secondary antibody (Rhodamine)
Specificity Murine IgG1
Cross Reactivity To be determined by end-user
Applications ELISA, FC, IF
Immunogen Rabbit anti-mouse IgG1 (Rhodamine) was raised in rabbit using murine IgG1 heavy chain as the immunogen.

Images

Dot Blot analysis using Rabbit anti Mouse IgG1 (rhodamine) (43C-CB5122)

Dot Blot of Rhodamine Conjugated Rabbit-anti-Mouse IgG1. Antigen: Mouse IgG1. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Rabbit-a-Mouse IgG1 secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.

Specifications

Host Rabbit
Method of Purification Rabbit anti Mouse IgG1 (Rhodamine) was purified by affinity chromatography.
Tag/Conjugate Rhodamine
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).

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Images

  • Dot Blot analysis using Rabbit anti Mouse IgG1 (rhodamine) (43C-CB5122) | Dot Blot of Rhodamine Conjugated Rabbit-anti-Mouse IgG1. Antigen: Mouse IgG1. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Rabbit-a-Mouse IgG1 secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.

Availability: In stock

Price: $295.00
Size: 1 mg
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