Rabbit anti Mouse IgG2b (FITC) (43C-CB5135)

Rabbit anti Mouse IgG2b secondary antibody (FITC)

Synonyms Rabbit anti Mouse Immunoglobulin G2b secondary antibody (FITC)
Specificity Murine IgG2b
Cross Reactivity To be determined by end-user
Applications ELISA, FC, IF
Immunogen Rabbit anti-mouse IgG2b (FITC) was raised in rabbit using murine IgG2b heavy chain as the immunogen.

Images

Western blot analysis using Rabbit anti Mouse IgG2b (FITC) (43C-CB5135)

Western Blot of Mouse IgG2b (Gamma 2b chain) Fluorescein Conjugated antibody. Lane 1: Mouse IgG2b. Lane 2: None. Load: 50 ng per lane. None. Fluorescein rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 55 kDa, 55 kDa for Mouse IgG2b. Other band(s): Light Chain.

Specifications

Host Rabbit
Method of Purification Rabbit anti Mouse IgG2b (FITC) was purified by affinity chromatography.
Tag/Conjugate FITC
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).

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Images

  • Western blot analysis using Rabbit anti Mouse IgG2b (FITC) (43C-CB5135) | Western Blot of Mouse IgG2b (Gamma 2b chain) Fluorescein Conjugated antibody. Lane 1: Mouse IgG2b. Lane 2: None. Load: 50 ng per lane. None. Fluorescein rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min at RT. Size: 55 kDa, 55 kDa for Mouse IgG2b. Other band(s): Light Chain.

Availability: In stock

Price: $295.00
Size: 1 mg
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