Rabbit IgG (Fab) (31C-CH1110)

Purified Rabbit IgG (Fab)

Synonyms Rabbit Immunoglobulin G (Fab)
Species Rabbit
Applications User optimized

Images

SDS-PAGE analysis of Rabbit IgG (Fab) (31C-CH1110)

Lane M: 3 uL MW Marker. Lane 1: Reduced Rabbit IgG Whole Molecule . Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

Specifications

Method of Purification Rabbit IgG (Fab) was purified by affinity chromatography.
Form & Buffer Supplied as a liquid in 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.2, with 0.01% NaN3 as a preservative.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope — the antigen-binding site — at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens.

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Images

  • SDS-PAGE analysis of Rabbit IgG (Fab) (31C-CH1110) | Lane M: 3 uL MW Marker. Lane 1: Reduced Rabbit IgG Whole Molecule . Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

Availability: In stock

Price: $265.00
Size: 5 mg
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