RABIF protein (His tag) (80R-1454)
Purified recombinant Human RABIF protein
|Synonyms||MSS4 protein, Rab-interacting factor protein, RASGRF3 protein, Guanine nucleotide exchange factor MSS4, RASGFR3 protein|
Coomassie Blue stained SDS-PAGE of RABIF protein (His tag) (80R-1454)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-123 amino acids: MGSSHHHHHH SSGLVPRGSH MEPAEQPSELVSAEGRNRKA VLCQRCGSRV LQPGTALFSR RQLFLPSMRK KPALSDGSNP DGDLLQEHWL VEDMFIFENV GFTKDVGNIK FLVCADCEIG PIGWHCLDDK NSFYVALERV SHE|
|Purity||> 85% pure|
|Molecular Weight||16.0 kDa (143aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||RABIF, also known as guanine nucleotide exchange factor MSS4, is a guanine-nucleotide-releasing protein that may participate in vesicular transport. This protein stimulates GTP-GDP exchange in Sec4 and Rab and binds to a subset of genetically related Rab proteins. Recombinant human RABIF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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