RAF1 antibody (20R-2976)

Rabbit polyclonal RAF1 antibody

Synonyms Polyclonal RAF1 antibody, Anti-RAF1 antibody, RAF1 antibody, v-raf-1 murine leukemia viral oncogene homolog 1 antibody, BOS_21199 antibody, c-mil protein antibody, c-Raf antibody, CRAF antibody, Craf1 antibody, Craf1 transforming antibody, MIL proto-oncogene serine/threonine-protein kinase antibody, murine leukemia viral (v-raf-1) oncogene homolog 1 antibody, murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV) antibody, murine sarcoma 3611 oncogene 1 antibody, NS5 antibody, Oncogene RAF1 antibody, proto-oncogene c-RAF antibody, RAF-1 antibody, Raf kinase antibody, effector of Ras antibody, raf proto-oncogene serine/threonine protein kinase antibody, RAF proto-oncogene serine/threonine-protein kinase antibody, sarcoma 3611 oncogene antibody, serine/threonine protein kinase antibody, serine/threonine protein kinase RAF1 antibody, v-Raf antibody, v-raf-1 leukemia viral oncogene 1 antibody, v-raf-leukemia viral oncogene 1 antibody
Cross Reactivity Human, Mouse, Rat
Applications WB
Immunogen RAF1 antibody was raised in Rabbit using a synthetic target peptide as the immunogen

Images

Western Blot analysis using RAF1 antibody (20R-2976)

Specifications

Host Rabbit
Isotype IgG
Method of Purification RAF1 antibody was purified by Protein A affinity chromatography
Form & Buffer Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Usage & Assay Information

Usage Recommendations WB: 1:2000

Storage & Safety

Storage Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles

General Information

Biological Significance RAF is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. The mammalian Raf family of serine/threonine kinases consists of three highly conserved members: A-Raf, B-Raf, and Raf-1 (or c-Raf-1). Each isoform has three conserved regions in common: CR1, CR2, and CR3. Raf isoforms vary in their cell-specific expression and potency of kinase activity. Cytosolic C-Raf ubiquitously expressed in adult tissues, with highest expression in muscle, cerebellum, and fetal brain. Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites. Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events.

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Images

  • Western Blot analysis using RAF1 antibody (20R-2976) |

Availability: In stock

Price: $295.00
Size: 100 ul
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