RAP1B protein (His tag) (80R-1649)

Purified recombinant Human RAP1B protein

Synonyms K-REV protein, RAPB 1 protein, RAP1B, DKFZp586H0723 protein, RAPB 1, RAPB-1, RAL1B, RAPB-1 protein, Ras-related protein Rap-1b protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of RAP1B protein (His tag) (80R-1649)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-181 amino acids: MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD LVRQINRKTP VPGKARKKSS C
Expression System E.coli
Purity > 90% pure
Molecular Weight 22.6 kDa (201aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 5mM DTT and 20% glycerol.
Concentration 0.25 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance RAP1B belongs to a superfamily of RAS-like small GTP-binding proteins involved in cell signaling. It possesses intrinsic GTPase activity and is ubiquitously expressed in mammalian tissues. The putative effector domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Recombinant human RAP1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of RAP1B protein (His tag) (80R-1649) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $340.00
Size: 50 ug
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