RAP1B protein (His tag) (80R-1649)
Purified recombinant Human RAP1B protein
|Synonyms||K-REV protein, RAPB 1 protein, RAP1B, DKFZp586H0723 protein, RAPB 1, RAPB-1, RAL1B, RAPB-1 protein, Ras-related protein Rap-1b protein|
Coomassie Blue stained SDS-PAGE of RAP1B protein (His tag) (80R-1649)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-181 amino acids: MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD LVRQINRKTP VPGKARKKSS C|
|Purity||> 90% pure|
|Molecular Weight||22.6 kDa (201aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 5mM DTT and 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||RAP1B belongs to a superfamily of RAS-like small GTP-binding proteins involved in cell signaling. It possesses intrinsic GTPase activity and is ubiquitously expressed in mammalian tissues. The putative effector domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Recombinant human RAP1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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