RBM8A protein (His tag) (80R-1748)
Purified recombinant Human RBM8A protein
|Synonyms||ZRNP1 protein, MDS014 protein, RBMA 8 protein, RBM8 protein, ZNRP protein, Y14 protein, RBMA-8 protein, RBMA-8, RBM8A, RNA-binding protein 8A protein, BOV-1C protein, BOV-1A; BOV-1B protein, RBM8B protein, RBMA 8|
Coomassie Blue stained SDS-PAGE of RBM8A protein (His tag) (80R-1748)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-174 amino acids: MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRRLEHHHH HH|
|Purity||> 90% pure|
|Molecular Weight||20.9 kDa(182aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||RBM8A is a member of the EJC (The exon junction complex) that is involved in mRNA export, cytoplasmic localization and nonsense mediated mRNA decay. This protein has the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. Although it shuttles to the cytoplasm, it is predominantly detected in the nucleus and is colocalized with oskar mRNA at the posterior pole of the cell. Recombinant human RBM8A protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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