RELA antibody (10R-1515)

Mouse monoclonal RELA antibody

Synonyms Monoclonal RELA antibody, Anti-RELA antibody, Reticuloendotheliosis viral oncogene homolog A antibody, Anti RELA antibody, RELA antibody, Reticuloendotheliosis viral oncogene homolog A antibody
Specificity Human RELA
Cross Reactivity Human, Rat. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen RELA antibody was raised in mouse using recombinant Human V-Rel Reticuloendotheliosis Viral Oncogene Homolog A,Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3, P65 (Avian)

Images

Immunofluorescent staining using RELA antibody (10R-1515)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-RELA antibody.

Specifications

Host Mouse
Clone 340C1a
Isotype IgG1
Method of Purification RELA antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer RELA antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex.

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Images

  • Immunofluorescent staining using RELA antibody (10R-1515) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-RELA antibody.
  • Western Blot analysis of immunized recombinant protein using RELA antibody (10R-1515)

Availability: In stock

Price: $190.00
Size: 50 ug
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