TMB or 3,3’,5,5’-Tetramethylbenzidine is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in ELISA.

TMB is a substrate for horseradish peroxidase, in the presence of HRP it develops a permanent, insoluble, dark blue reaction product. This is extremely useful for visualizing the presence of HRP in samples in a variety of assays.

TMB is the most popular chromogenic substrate for HRP detection in ELISA and is available in several formats. Fitzgerald Industries offers a variety of TMB substrates depending on your needs including TMB Peroxidase Substrate, TMB ELISA Substrate, TMB for Histochemistry and TMB Weakener for the dilution of TMB Substrate in immunoassays.


Check out our full list of substrates on our website, or visit our homepage to search for more products!