RHOA protein (His tag) (80R-1608)
Purified recombinant Human RHOA protein
|Synonyms||Ras homolog gene family protein, RHOH12, RHO12 protein, ARH12 protein, ARHA protein, member A protein|
Coomassie Blue stained SDS-PAGE of RHOA protein (His tag) (80R-1608)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-190 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC|
|Purity||> 90% pure|
|Molecular Weight||25.7 kDa(227aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila). Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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