RhoB protein (His tag) (80R-1813)
Purified recombinant Human RhoB protein
|Synonyms||Rho-related GTP-binding protein RhoB protein, MST081 protein, ARHB protein, RHOH6 protein, MSTP081 protein, ARH6 protein|
Coomassie Blue stained SDS-PAGE of RhoB protein (His tag) (80R-1813)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-193 amino acids: MGSSHHHHHH SSGLVPRGSH MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC|
|Purity||> 90% pure|
|Molecular Weight||23.9 kDa (213aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 2mM DTT, 20% glycerol and 0.2mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Rho-related GTP-binding protein RhoB, also known as RhoB, is a member of the Rho GTP-binding protein family. RhoB mediates apoptosis in neoplastically transformed cells after DNA damage. It plays a negative role in tumorigenesis as deletion causes tumor formation. Recombinant human RhoB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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