RhoG protein (His tag) (80R-2335)

Purified recombinant Human RhoG Protein (His tag)

Synonyms Rho-related GTP-binding RhoG protein, ARHG protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of RhoG protein (His tag) (80R-2335)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMQS IKCVVVGDGA VGKTCLLICY TTNAFPKEYI PTVFDNYSAQ SAVDGRTVNL NLWDTAGQEE YDRLRTLSYP QTNVFVICFS IASPPSYENV RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAVR YLECSALQQD GVKEVFAEAV RAVLNPTPIK RGRSC
Expression System E.coli
Purity > 90% pure
Molecular Weight 25.2 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20 mM Tris-HCl buffer, pH 8.0, 40% glycerol, 5mM DTT, 200mM NaCl
Concentration 250 ug/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1-2 weeks). Aliquot and store at -20 deg C to -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance RhoG is a member of the Rac subfamily of the Rho family of small G proteins. RhoG is a small monomeric GTP-binding protein (G protein), and is an important component of many intracellular signalling pathways. This protien are required for the formation of membrane ruffles during macropinocytosis. It plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. Like most small G proteins RhoG is involved in a diverse set of cellular signalling mechanisms. In mammalian cells these include cell motility, gene transcription, endocytosis, neurite outgrowth, protection from anoikis and regulation of the neutrophil NADPH oxidase. Recombinant human RhoG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of RhoG protein (His tag) (80R-2335) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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