RNMT protein (His tag) (80R-1825)
Purified recombinant RNMT protein
|Synonyms||MET protein, HCMT1a protein, MET protein, mRNA (guanine 7)methyltransferase protein, RG7MT1 protein, hcm1p protein, hCMT1 protein, hypothetical protein KIAA0396 protein, mRNA (guanine N(7)) methyltransferase protein, RNMT protein, hCMT1c protein, OTTHUMP00000162509 protein, mRNA cap methyltransferase protein, mRNA cap guanine-N7 methyltransferase protein, hMet protein, RNA guanine 7 methyltransferase protein, DKFZp686H1252 protein, KIAA0398 protein, RNA guanine-7-methyltransferase protein, EC 22.214.171.124 protein|
Coomassie Blue stained SDS-PAGE of RNMT protein (His tag) (80R-1825)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-476 amino acids: MGSSHHHHHH SSGLVPRGSH MANSAKAEEY EKMSLEQAKA SVNSETESSF NINENTTASG TGLSEKTSVC RQVDIARKRK EFEDDLVKES SSCGKDTPSK KRKLDPEIVP EEKDCGDAEG NSKKRKRETE DVPKDKSSTG DGTQNKRKIA LEDVPEKQKN LEEGHSSTVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSVKQCQQRY EDMKNRRDSE YIFSAEFITA DSSKELLIDK FRDPQMCFDI CSCQFVCHYS FESYEQADMM LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP YPANESSKLV SEKVDDYEHA AKYMKNSQVR LPLGTLSKSE WEATSIYLVF AFEKQQ|
|Purity||> 90% pure|
|Molecular Weight||57.0 kDa (496aa)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 2mM DTT, 20% glycerol and 100mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||RNMT (mRNA cap guanine-N7 methyltransferase), also known as MET, RG7MT1 or hCMT1c, is a widely expressed nuclear protein that belongs to the mRNA cap methyltransferase family. Cap-dependent mRNA translation requires the methylation of the mRNA guanosine cap by RNMT. RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thereby producing AdoHyc (S-adenosylhomocysteine) and m7GpppN terminated RNA. Recombinant human RNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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