RPA1 antibody (10R-1208)

Mouse monoclonal RPA1 antibody

Synonyms Monoclonal RPA1 antibody, Anti-RPA1 antibody, RPA70 antibody, REPA1 antibody, RF-A antibody, RPA 1 antibody, Replication protein A1 antibody, RPA-1 antibody, RP-A antibody, RPA 1, RPA-1, HSSB antibody, RPA1
Specificity Human RPA1
Cross Reactivity Human. Other species not studied
Applications Dot Blot, FC, WB
Immunogen RPA1 antibody was raised in mouse using recombinant Replication Protein A1, 70Kda (Rpa1)

Images

Flow Cytometric assay using RPA1 antibody (10R-1208)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RPA1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone RPA15I373
Isotype IgG1
Method of Purification RPA1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer RPA1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Replication protein A1 functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange

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Images

  • Flow Cytometric assay using RPA1 antibody (10R-1208) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RPA1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using RPA1 antibody (10R-1208)

Availability: In stock

Price: $190.00
Size: 50 ug
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