RPS3 protein (His tag) (80R-2007)

Recombinant human RPS3 protein (His tag)

Synonyms RPS-3 protein, 40S ribosomal protein S3 protein, RPS-3, RPS3, RPS 3 protein, RPS 3
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of RPS3 protein (His tag) (80R-2007)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-243 amino acids: MGSSHHHHHH SSGLVPRGSH MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPTGKIGP KKPLPDHVSI VEPKDEILPT TPISEQKGGK PEPPAMPQPV PTA
Expression System E.coli
Purity > 90% pure
Molecular Weight 28.8 kDa (263aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations RPS3 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance 40S ribosomal protein S3, also known as RPS3, belongs to the S3P family of ribosomal proteins. RPS3 mediates the formation of the mRNA binding site 3' of the codon in the decoding site. In addition, RPS3 is involved in DNA damage recognition as shown by its affinity for abasic sites and 7, 8-dihydro-8-oxoguanine residues and its interaction with human base excision repair proteins OGG1 and Ref-1. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. Recombinant human RPS3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of RPS3 protein (His tag) (80R-2007) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 50 ug
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