RSV antibody (FITC) (60-R41)
Goat polyclonal RSV antibody (FITC) conjugated
|Synonyms||Polyclonal RSV antibody, Anti-RSV antibody, Respiratory Syncytial Virus antibody|
|Cross Reactivity||To be determined by end-user|
|Immunogen||RSV antibody (FITC) was raised in goat using human RSV isolate as the immunogen.|
|Form & Buffer||Protein A purified in 10mM PBS, pH 7.2, with 10 mg/ml BSA and 0.1% NaN3.|
|Concentration||Typically 4-5 mg/ml|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biohazard Information||This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.|
|Biological Significance||Human respiratory syncytial virus (RSV) is a virus that causes respiratory tract infections. It is the major cause of lower respiratory tract infection and hospital visits during infancy and childhood. There is a prophylactic medication (not a vaccine) for premature (<35 Wks) infants and infants with congenital heart disease (CHD) or bronchopulmonary dysplasia (BPD). Treatment is limited to supportive care, including oxygen.|
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|Submitted By||Oliver Wicht, (CIB, NIPBE)|
|Date||14th July 2016|
|Application product used in||Flow Cytometry|
|Usage||1:100 to 1:3200 dilution series using fresh human PBMCs.|
|Comments||High concentrations of antibody gives very robust signal; cheap product. Cells with low expression of antigen or early during infection can be missed due to high background. Infection of RSV was verified by two step stainings using alternative antibodies. With this FITC-conjugated antibody, we detect less positive cells than expected. Uninfected control cells are always necessary due to the high background. The antibody works better on homogeneous cell populations like immortalized cell lines.
Review courtesy of Biocompare.