RUVBL1 antibody (10R-1392)

Mouse monoclonal RUVBL1 antibody

Synonyms Monoclonal RUVBL1 antibody, Anti-RUVBL1 antibody, TIP49A antibody, RuvB (E coli homolog)-like 1 antibody, RUVBL 1 antibody, TIP49 antibody, NMP238 antibody, RUVBL1, RUVBL 1, RUVBL-1 antibody, RVB1 antibody, RUVBL-1, ECP54 antibody
Specificity Human RUVBL1
Cross Reactivity Human. Other species not studied
Applications Dot Blot, FC, ICC, WB
Immunogen RUVBL1 antibody was raised in mouse using recombinant Human Ruvb-Like 1 (E. Coli) (Ruvbl1)

Images

Flow Cytometric assay using RUVBL1 antibody (10R-1392)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RUVBL1 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone 2943C1a
Isotype IgG2b
Method of Purification RUVBL1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer RUVBL1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair.

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Images

  • Flow Cytometric assay using RUVBL1 antibody (10R-1392) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RUVBL1 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using RUVBL1 antibody (10R-1392)
  • Immunofluorescent staining using RUVBL1 antibody (10R-1392) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-RUVBL1 antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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