SAE1 protein (T7 tag) (80R-1373)
Purified recombinant Human SAE1 protein (T7 tag)
|Synonyms||Ubiquitin-like 1-activating enzyme E1A protein, SAE 1 protein, SUA 1 protein, Ubiquitin like protein SUMO 1 activating enzyme, SUA1 protein, UBLE1A protein, SUMO1 activating enzyme subunit 1 protein, SAE 1 protein, SAE1, UBLE 1A protein, SAE-1, SAE-1 protein, AOS 1 protein, HSPC 140 protein, SAE 1, HSPC140 protein, UBL E1A protein, AOS1 protein|
Coomassie Blue stained SDS-PAGE of SAE1 protein (T7 tag) (80R-1373)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-346 amino acids: MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK|
|Purity||> 90% pure|
|Molecular Weight||42.2 kDa (378aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the UBL1 conjugation pathway. Proteins conjugated to Ub are marked for progressive degradation by the 26S Proteasome. SAE1, which is a dimeric enzyme, functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. This protein can bind with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. Recombinant human SAE1 protein, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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