Secondary Antibody

A Secondary antibody is a polyclonal or monoclonal antibody that binds to primary antibodies or antibody fragments, such as the Fc or Fab regions.

Secondary antibody binding

Secondary antibodies are generated by immunizing a host animal with an antibody from a different species. For example, anti-mouse antibodies are raised by injecting specific purified mouse antibody into an animal other than a mouse. Goat, donkey, sheep, chicken and rabbit are the most commonly used host species for raising secondary antibodies, though others are available.

Secondary antibodies are often labelled with probes such as Fluorescein (FITC), biotin, (biotinylated antibody), horseradish peroxidase (HRP conjugated antibody) and others that make them useful for detection, purification or sorting applications, they are utilized in many applications including ELISA, Immunopurification, Western blotting, Immunohistochemistry, Immunoassays, Flow Cytometry and more. Indirect detection using secondary antibodies by binding to a primary antibody which in turn binds to the target greatly increases sensitivity and signal amplification, this has valuable applications in procedures such as quantitative and qualitative assays and purification.

Fitzgerald Industries has a vast range of monoclonal and polyclonal secondary antibodies, as both unconjugated secondary antibodies and conjugated secondary antibodies expressed in all commonly used host species. We supply antibodies to commonly used immunoglobulins such as IgG antibodies, IgM antibodies, IgA antibodies, and IgE antibodies which are specific for a broad range of species including Anti-Human, Anti-Mouse, Anti-Rat, Anti-Rabbit, Anti-Goat and Anti-Chicken.

Check out our full range of secondary antibodies or contact us with your specific requirements.