Mice are one of the most popular host animals used in the production of monoclonal primary antibodies. During immunological assays such as ELISA, Immunoblotting, Immunostaining, Immunohistochemistry, Immunocytochemistry and others, indirect detection using secondary antibodies binding to a primary antibody which in turn binds to the target greatly increases sensitivity and signal amplification, this has many applications in both research and diagnostic tests such as HIV, cancer, cardiac markers etc, as well as in purification procedures.
Fitzgerald Industries offers a huge range of mouse secondary antibodies expressed in most commonly utilized host animals with high specificity for the major mice immunoglobulin classes including IgG, IgE, IgM, IgA and their subclasses and fragments. They are available in unlabelled format as well as conjugates of useful tags such as alkaline phosphatase (AP), horseradish peroxidase (HRP), FITC, and biotin. Some of our top selling anti-Mouse immunoglobulin antibodies are:
Rat anti Mouse IgG is supplied as a raw serum and is available as a bulk anti mouse IgG.
Rat anti Mouse IgE is murine specific and ideal for ELISA assays.
We also have a number of conjugated mouse secondary antibodies such as:
Goat anti Mouse IgG (HRP) was purified by affinity chromatography and conjugated to horseradish peroxidase making it an ideal choice for ELISA.
Goat anti Mouse IgG + IgM (H + L) (Alk Phos) this IgG/IgM mix is conjugated with alkaline phosphatase and is an excellent choice for multiple assays like ELISA, IHC, Western Blotting and more.