Conjugated Secondary antibody
Conjugation or labelling of an antibody with a fluorescent dye, ferromagnetic bead, agarose bead etc is a powerful tool for allowing fluorogenic or chromogenic visual detection of the antibody. In the case of conjugated secondary antibodies, the antibody is often labelled with a flourochrome or chromogen so that when it attached to a primary antibody of interest it greatly increases its visibility and detection rates.
The most common detection systems for conjugated secondary antibodies are colorimetric or fluorescent. Colorimetric assays are typically based on the use of alkaline phosphatase (AP) or horseradish peroxidase (HRP) or its derivatives. The biotin avidin (streptavidin) conjugate binding system is often used to amplify the colorimetric signal for alkaline phosphatase or HRP. The most common fluorescent assays utilize Fluorescein (FITC) or Phycoerythrin (R-PE).
Fitzgerald Industries offers a huge range of conjugated secondary antibodies from a wide selection of host species and conjugated to all commonly utilised tags including Fluorescein (FITC), biotin, horseradish peroxidase (HRP), Agarose, Alexa fluor, CY3, Rhodamine and many more. We supply conjugated versions of most immunoglobulins and their subclasses as well as specific regions such as fab fragments and antibody chains.
Some examples of our most popular conjugated secondary antibodies include Rabbit anti Sheep IgG (biotin) for use in ELISA, IHC or WB. Mouse anti Human IgG1 (HRP) for use in ELISA and purified Goat anti Monkey IgG (HRP), and Rabbit anti Bovine IgG (Texas Red) for FACS, ELISA and immunofluorescence assays.