SEP15 protein (His tag) (80R-2811)

Purified recombinant SEP15 protein (His tag)

Synonyms 15 kDa selenoprotein protein, SEP15, SC96C protein, SC96C
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of SEP15 protein (His tag) (80R-2811)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 29-165 aa: MGSSHHHHHH SSGLVPRGSH MGSVSAFGAE FSSEACRELG FSSNLLCSSC DLLGQFNLLQ LDPDCRGCCQ EEAQFETKKL YAGAILEVCG CKLGRFPQVQ AFVRSDKPKL FRGLQIKYVR GSDPVLKLLD DNGNIAEELS ILKWNTDSVE EFLSEKLERI
Expression System E.coli
Purity > 95% pure
Molecular Weight 17.7 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Concentration 500 ug/ml

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance SEP15 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Studies in mouse suggest that this selenoprotein may have redox function and may be involved in the quality control of protein folding. This gene is localized on chromosome 1p31, a genetic locus commonly mutated or deleted in human cancers. Recombinant human SEP15 (SC96C) protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • SDS-PAGE analysis of SEP15 protein (His tag) (80R-2811) | Figure annotation denotes ug of protein loaded and % gel used.
  • SDS-PAGE analysis of SEP15 protein (His tag) (80R-2811) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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