SEPT5 protein (His tag) (80R-1879)
Purified recombinant 38596 protein
|Synonyms||CDCREL-1 protein, SEPT-5, PNUTL1 protein, CDCREL protein, SEPT-5 protein, SEPT5, SEPT 5 protein, H5 protein, SEPT 5, CDCREL1 protein|
Coomassie Blue stained SDS-PAGE of SEPT5 protein (His tag) (80R-1879)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-369 amino acids: MGSSHHHHHH SSGLVPRGSH MGSMSTGLRY KSKLATPEDK QDIDKQYVGF ATLPNQVHRK SVKKGFDFTL MVAGESGLGK STLVHSLFLT DLYKDRKLLS AEERISQTVE ILKHTVDIEE KGVKLKLTIV DTPGFGDAVN NTECWKPITD YVDQQFEQYF RDESGLNRKN IQDNRVHCCL YFISPFGHGL RPVDVGFMKA LHEKVNIVPL IAKADCLVPS EIRKLKERIR EEIDKFGIHV YQFPECDSDE DEDFKQQDRE LKESAPFAVI GSNTVVEAKG QRVRGRLYPW GIVEVENQAH CDFVKLRNML IRTHMHDLKD VTCDVHYENY RAHCIQQMTS KLTQDSRMES PIPILPLPTP DAETEKLIRM KDEELRRMQE MLQRMKQQMQ DQ|
|Purity||> 90% pure|
|Molecular Weight||45.2 kDa (392aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 40% glycerol, 0.3M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SEPT5, also known as septin-5, belongs to the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Recombinant human SEPT5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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