SEPX1 protein (His tag) (80R-1497)
Purified recombinant Human SEPX1 protein
|Synonyms||SEPX-1, SELX protein, SEPX1, MSRB1 protein, U95C, HSPC270 protein, SELR protein, SEPX-1 protein, SEPX 1, SEPX 1 protein, MSRB1 protein, Selenoprotein X protein, Methionine-R-sulfoxide reductase B1 protein|
Coomassie Blue stained SDS-PAGE of SEPX1 protein (His tag) (80R-1497)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-116 amino acids: MGSSHHHHHH SSGLVPRGSH MSFCSFFGGE VFQNHFEPGV YVCAKCGYEL FSSRSKYAHS SPWPAFTETI HADSVAKRPE HNRSEALKVS CGKCGNGLGH EFLNDGPKPG QSRFCIFSSS LKFVPKGKET SASQGH|
|Purity||> 90% pure|
|Molecular Weight||14.8 kDa(136aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl Buffer, pH 7.5, containing 1mM DTT, 0.1mM PMSF, 2mM EDTA, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SEPX1, also known as Methionine sulfoxide reductase B1 (MSRB1), is a selenoprotein. Methionine sulfoxide reductases (MSRs) catalyze reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS generates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). Two distinct enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO. In bacteria, the selenocystein (Sec/U) element is located immediately following the UGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys. Recombinant human SEPX1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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