SERCA1 antibody (10R-S129a)

Mouse monoclonal SERCA1 antibody

Synonyms Monoclonal SERCA1 antibody, Anti-SERCA1 antibody, SERCA1 ATPase antibody, SER Calcium 2 ATPase (SERCA 1) antibody, Sarcoplasmic/Endoplasmic Reticulum Calcium 1 ATPase (SERCA 1) antibody, Anti SERCA1 antibody, Sarcoplasmic/Endoplasmic Reticulum Calcium 1 ATPase (SERCA 1) antibody, SERCA1 antibody, SER Calcium 2 ATPase (SERCA 1) antibody, SERCA1 ATPase antibody
Specificity Human SERCA1
Cross Reactivity To be determined by end-user
Applications IF, WB
Immunogen SERCA1 antibody was raised in mouse using purified rabbit skeletal muscle sarcoplasmic reticulum as the immunogen.

Images

Immunohistochemical staining using SERCA1 antibody (10R-S129a)

Immunohistochemistry was performed on normal deparaffinized Human heart tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were probed at a dilution of 1:200 with SERCA1 antibody or without antibody (negative control) overnight at 4 deg C in a humidified chamber. Tissues were washed well with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Specifications

Host Mouse
Clone VE121G9
Isotype IgG1
Form & Buffer Ascites diluted in PBS with 0.05% NaN3.
Concentration Batch dependent - please inquire should you have specific requirements

Usage & Assay Information

Usage Recommendations IF: 1:500, WB: 1:2,500

Storage & Safety

Storage Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Antigen is purified rabbit skeletal muscle sarcoplasmic reticulum. This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.

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Images

  • Immunohistochemical staining using SERCA1 antibody (10R-S129a) | Immunohistochemistry was performed on normal deparaffinized Human heart tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were probed at a dilution of 1:200 with SERCA1 antibody or without antibody (negative control) overnight at 4 deg C in a humidified chamber. Tissues were washed well with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Availability: In stock

Price: $625.00
Size: 100 ul
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