Sheep anti Bovine IgG1 (HRP) (43R-IS003hrp)
Sheep anti Bovine IgG1 secondary antibody (HRP)
Overview
Overview
| Synonyms | Bovine Immuonglobulin G2 antibody (HRP), Cow IgG2 antibody (HRP) |
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| Specificity | Bovine |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, ICC, IHC, WB |
Specifications
| Host | Sheep |
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| Isotype | IgG |
| Method of Purification | Sheep anti Bovine IgG1 (HRP) was purified by affinity chromatography. |
| Tag/Conjugate | HRP |
| Form & Buffer | PBS with 0.1% Pro-Clean 400. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, IHC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000 |
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Storage & Safety
| Storage | Store at 4 deg C. |
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General Information
| Biological Significance | IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria. |
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References
References
Bovine immune response to papillomatous digital dermatitis (PDD)-associated spirochetes is skewed in isolate reactivity and subclass elicitation
Veterinary Immunology and Immunopathology
Volume: 130
Issue: 3-4
Page: 256-261
DOI: 10.1016/j.vetimm.2009.02.010
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