SHH protein (His tag) (80R-1339)
Purified recombinant Human SHH protein
|Synonyms||MCOPCB5 protein, Sonic hedgehog protein HHG 1 protein, Sonic Hedgehog (Drosophila) homolog protein, HHG1 protein, Holoprosencephaly 3 protein, SMMC I protein, , TPTPS protein, TPT protein, HLP3 protein, HPE3 protein, SMMCI protein, Sonic hedgehog homolog protein, HLP 3 protein, SMMCI protein, HPE 3 protein, SHH protein, sonic hedgehog homolog (Drosophila) protein|
Coomassie Blue stained SDS-PAGE of SHH protein (His tag) (80R-1339)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||24-197 amino acids: MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKISRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH|
|Purity||> 95% pure|
|Molecular Weight||20.7 kDa (183aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SHH, also known as Sonic hedgehog protein, is one of three proteins in the mammalian signaling pathway family called hedgehog. It plays a key role in regulating vertebrate organogenesis, such as in the growth of digits on limbs and organization of the brain. It controls cell division of adult stem cells and has been implicated in development of some cancers. Recombinant human SHH protein, fused to His-tag at C-terminus was expressed in E. coli and purified by using conventional chromatography techniques.|
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