SKP2 antibody (10R-2070)
Mouse monoclonal SKP2 antibody
|Synonyms||Monoclonal SKP2 antibody, Anti-SKP2 antibody, p45 antibody, SKP 2, SKP-2 antibody, SKP-2 antibody, SKP2, SKP-2, SKP 2 antibody, SKP 2 antibody, S phase kinase associated protein 2 antibody|
|Immunogen||SKP2 antibody was raised in Mouse using a purified recombinant fragment of SKP2(aa1-130) expressed in E. coli as the immunogen.|
Western Blot analysis using SKP2 antibody (10R-2070)
Western Blot showing SKP2 antibody used against truncated Trx-SKP2 recombinant protein (1) and GST-SKP2 (aa1-130) recombinant protein (2).
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SKP2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs. SKP2 is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19).|
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