SMAD1/5 antibody (10R-8575)

Mouse monoclonal SMAD1/5 antibody

Synonyms Monoclonal SMAD1/5 antibody, Anti-SMAD1/5 antibody, SMAD1 antibody, SMAD family member 1 antibody, BMP pathway effector antibody, BMP signal transducer SMAD1 antibody, BOS_16283 antibody, BSP1 antibody, BSP-1 antibody, dwarfin-A antibody, dwf-A antibody, EGK_16135 antibody, fb39h09 antibody, hypothetical protein antibody, jv41 antibody, JV4-1 antibody, madh1 antibody, MAD homolog 1 antibody, MAD homolog 1 (Drosophila) antibody, MAD homolog1 (mothers against decapentaplegic antibody, Drosophila) antibody, MAD (mothers against decapentaplegic antibody, Drosophila) homolog 1 antibody, MAD antibody, mothers against decapentaplegic homolog 1 antibody, Madr1 antibody, mad-related protein 1 antibody, Mlp1 antibody, mMad1 antibody, mothers against decapentaplegic homolog 1 antibody, mothers against decapentaplegic-like protein 1 antibody, mothers against DPP homolog 1 antibody, mothers-against-DPP-related 1 antibody, MusMLP antibody, SMA- and MAD-related protein 1 antibody, SMAD-1 antibody, SMAD 1 antibody, SMAD antibody, mothers against DPP homolog 1 antibody, TGF-beta signaling protein 1 antibody, transforming growth factor-beta signaling protein 1 antibody, transforming growth factor-beta-signaling protein 1 antibody, Xmad1 antibody, XSMAD1 antibody
Cross Reactivity Human, Rat
Applications ELISA, WB
Immunogen SMAD1/5 antibody was raised in Mouse using a recombinant human protein purified from E.coli (His-SMAD1) as the immunogen

Images

Western Blot analysis using Smad1/5 antibody (10R-8575)

Specifications

Host Mouse
Clone AF10B7
Isotype IgG2a kappa
Method of Purification SMAD1/5 antibody was purified by ammonium sulfate precipitation
Form & Buffer Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Usage & Assay Information

Usage Recommendations WB: 1:1000-2000

Storage & Safety

Storage Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles

General Information

Biological Significance In mammals, eight SMAD proteins have been identified to date. The SMAD family of proteins can be divided into three functional groups: the receptor-activated SMADs (R-SMADs), common mediator SMADs (Co-SMADs), and the inhibitory SMADs (I-SMADs). The R-SMADs are directly phosphorylated by the activated type I receptors on their C-terminal Ser-Ser-X-Ser (SSXS) motif and include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8. SMAD2 and SMAD3 are phosphorylated in response to TGF-beta and activin, whereas SMAD1, SMAD5, and SMAD8 are phosphorylated in response to BMP (Bone Morphogenetic Protein). This C-terminal phosphorylation allows R-SMAD binding to Co-SMAD, SMAD4, and translocation to the nucleus where they regulate TGF-beta target genes. SMAD6 and SMAD7 belong to the I-SMAD which bind to the type I receptor or SMAD4 and block their interaction with R-SMADs.

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Images

  • Western Blot analysis using Smad1/5 antibody (10R-8575) |

Availability: In stock

Price: $295.00
Size: 100 ul
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