SMAD3 antibody (10R-2212)

Mouse monoclonal SMAD3 antibody

Synonyms Monoclonal SMAD3 antibody, Anti-SMAD3 antibody, SMAD-3 antibody, SMAD 3 antibody, HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, MADH3 antibody, Mothers against decapentaplegic homolog 3 DKFZP586N0721 antibody, DKFZp686J10186 antibody, hMAD 3 antibody, hSMAD3 antibody, MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody, HST17436 antibody, JV15 2 antibody, JV152 antibody, MAD3 antibody, MADH 3 antibody, MGC60396 antibody, Mothers against DPP homolog 3 antibody, SMA and MAD related protein 3 antibody, SMAD3, SMAD 3, SMAD-3, SMAD 3 antibody, SMAD-3 antibody
Cross Reactivity Human, Mouse
Applications ELISA, IF, WB
Immunogen SMAD3 antibody was raised in Mouse using a purified recombinant fragment of human SMAD3 expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using SMAD3 antibody (10R-2212) (SMAD3 antibody)

Immunofluorescence analysis of NIH/3T3 cells using SMAD3 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Specifications

Host Mouse
Clone 5G11
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:200-1:1000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance SMAD3 belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. Small molecule inhibitors of Smad3 may have tremendous clinical potential in the treatment of pathological fibrotic diseases.

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Images

  • Immunofluorescent staining using Western Blot analysis using SMAD3 antibody (10R-2212) (SMAD3 antibody) | Immunofluorescence analysis of NIH/3T3 cells using SMAD3 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western Blot analysis using SMAD3 antibody (10R-2212) | Western Blot showing SMAD3 antibody used against A549 (1), Hela (2), Jurkat (3), PC-2 (4) and NIH/3T3 (5) cell lysate.

Availability: In stock

Price: $345.00
Size: 100 ul
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