SMAD4 antibody (10R-2234)

Mouse monoclonal SMAD4 antibody

Synonyms Monoclonal SMAD4 antibody, Anti-SMAD4 antibody, SMAD family member 4 antibody, JIP antibody, DPC4 antibody, MADH4 antibody, SMAD4, SMAD 4, SMAD-4, SMAD 4 antibody, SMAD-4 antibody
Cross Reactivity Human
Applications ELISA, IF, IHC, WB
Immunogen SMAD4 antibody was raised in Mouse using a purified recombinant fragment of human SMAD4 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using SMAD4 antibody (10R-2234) (SMAD4 antibody)

FC analysis of K562 cells using SMAD4 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 4G1C6
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

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Images

  • Flow Cytometric assay using Western Blot analysis using SMAD4 antibody (10R-2234) (SMAD4 antibody) | FC analysis of K562 cells using SMAD4 antibody (green) and negative control (purple).
  • Western Blot analysis using SMAD4 antibody (10R-2234) | Western Blot showing SMAD4 antibody used against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.
  • Immunohistochemical staining using SMAD4 antibody (10R-2234) | Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SMAD4 antibody with DAB staining.
  • Immunofluorescent staining using Western Blot analysis using SMAD4 antibody (10R-2234) (SMAD4 antibody) | Immunofluorescence analysis of NIH/3T3 cells using SMAD4 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
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