SOX4 antibody (10R-1607)

Mouse monoclonal SOX4 antibody

Synonyms Monoclonal SOX4 antibody, Anti-SOX4 antibody, SOX 4 antibody, SOX-4, EVI16 antibody, SOX 4, SOX-4 antibody, SOX4, SRY (sex determining region Y) box 4 antibody
Specificity Human SOX4
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen SOX4 antibody was raised in mouse using recombinant Human Sry (Sex Determining Region Y)-Box 4 (Sox4)

Images

Immunofluorescent staining using SOX4 antibody (10R-1607)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-SOX4 antibody.

Specifications

Host Mouse
Clone 154C4a
Isotype IgG2a
Method of Purification SOX4 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer SOX4 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

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Images

  • Immunofluorescent staining using SOX4 antibody (10R-1607) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-SOX4 antibody.
  • Western Blot analysis of immunized recombinant protein using SOX4 antibody (10R-1607)

Availability: In stock

Price: $395.00
Size: 50 ug
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