SP100 protein (His tag) (80-1387)

Purified recombinant SP100 protein (His tag)

Synonyms Nuclear autoantigen Sp100, SP100 Nuclear Antigen, Nuclear dot-associated Sp100 protein, Speckled 100 kDa protein
Species Human
Protein Type Recombinant

Specifications

Residues Amino acids1-474
Expression System E.coli
Purity > 90% pure
Method of Purification Purified using proprietary chromatographic techniques
Molecular Weight 57 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in PBS buffer with 2mM DTT, 1mM PMSF, 1mM EDTA and 0.02% SDS

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Together with PML, SP100 tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. SP100 functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. SP100 may also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis.
Expression SP100 is widely expressed in humans. Tissue and Cell Localization: Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines.
Implications in Disease SP100 is one of the major tumor suppressors involved in cellular differentiation. Mutations in expression have been linkedto multiple carcinomas as well as Primary Biliary Cirrhosis (PBC), a chronic disease that affects the bile ducts within the liver
Sequence Information SP100 is composed of 879 amino acids with a molecular weight of 100417 Da. It contains 2 HMG box DNA-binding domains, 1 HSR and 1 SAND domain. The HSR domain is important for the nuclear body targeting as well as for the dimerization.
Post-Translational Modifications Sumoylation of SP100 depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting. Ubiquitination occurs at Lys151.
Database Links Entrez Gene: 6672 Human, Omim: 604585 Human, SwissProt: P23497 Human, Unigene: 369056 Human

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